Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110846_3yl6 ACCTTACTCATAATGTCCCTGCAGGAGATTGG(+CAGTGT)GATCCTTTCCGTGGGACAGTAACTTTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605110844_iwua CCTTGACCATTATAATTTAAATGTGTTTGGAG(+CAGTGT)GTACCAGGCTCATGGGCATAATTTATAATAT NGG 10 8 17 8 24 0 100 5 10
    240605110842_x7wu AGTTGATATTATAAATTATGCCCATGAGCCTG(+CAGTGT)GTACCTCCAAACACATTTAAATTATAATGGT NGG 10 8 17 8 24 0 100 5 10
    240605110840_1bn7 TGAATCATTAGAACGCTAGAAGACAATATGGG(+CAGTGT)GTACCCCTTTACCAAGATTTTGAGAAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605110838_wb1c CAGTCATTCTTTCTCAAAATCTTGGTAAAGGG(+CAGTGT)GTACCCCATATTGTCTTCTAGCGTTCTAATG NGG 10 8 17 8 24 0 100 5 10
    240605110837_l307 AGGGATCCTATGACTGACCAACAAAAGTACAG(+CAGTGT)GTACCACCAGAGTCCAACTTGTTGAAACAAT NGG 10 8 17 8 24 0 100 5 10
    240605110836_58kq AATGCATTGTTTCAACAAGTTGGACTCTGGTG(+CAGTGT)GTACCTGTACTTTTGTTGGTCAGTCATAGGA NGG 10 8 17 8 24 0 100 5 10
    240605110834_30je TAATTTCTCATTACCCTCTGTAAACACAATGG(+CAGTGT)GTACCCTTTGTTTTCCTCTCCTAAAAATAAC NGG 10 8 17 8 24 0 100 5 10
    240605110832_e17r ACAAAGTTATTTTTAGGAGAGGAAAACAAAGG(+CAGTGT)GTACCCATTGTGTTTACAGAGGGTAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240605110831_ozw3 AATGTTGGGAGATCCCAGAAGCTATAGTGAAG(+CAGTGT)GTACCTGAAGGGCATTGCTAGTAGGATAGAG NGG 10 8 17 8 24 0 100 5 10