Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110830_uwha TCCCACTCTATCCTACTAGCAATGCCCTTCAG(+CAGTGT)GTACCTTCACTATAGCTTCTGGGATCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605110828_9pw6 CTACAGGGAGGGAATCAGGCCACACTGCTGAG(+CAGTGT)GTACCCTGTGGTTCCTTTATAAGCTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605110826_zlhc ACCAAACAAGAAGCTTATAAAGGAACCACAGG(+CAGTGT)GTACCTCAGCAGTGTGGCCTGATTCCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605110824_n7w3 TGACCTCAGCAAATCCTCCTTTTAACTCATGG(+CAGTGT)GTACCCAGACTCGAACTATTTCACAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240605110823_nw7x TGGTTGTGAATTGTGAAATAGTTCGAGTCTGG(+CAGTGT)GTACCCATGAGTTAAAAGGAGGATTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605110821_jujr AAACTTGGCTGACTTAGAACTGGAAGTTGCTG(+CAGTGT)GTACCCAGGCTGAAATCTCTTGCGCTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605110819_wg0c TCAAGAAGCCAGCGCAAGAGATTTCAGCCTGG(+CAGTGT)GTACCAGCAACTTCCAGTTCTAAGTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605110818_wj0g TGTGGATAACATATTATGTATATATGTAATGG(+CAGTGT)GTACCAGGCCAGGTTTATATTTTTTTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240605110816_v0cp ACGTCTAAAGAAAAAAATATAAACCTGGCCTG(+CAGTGT)GTACCCATTACATATATACATAATATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605110814_67l4 GCTCACAACCATGCATAACTCAAGTTTTAAGG(+CAGTGT)GATCCAGTGCTGACTTCTGGCCTCTGAGGGT NGG 10 8 17 8 24 0 100 5 10