Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110745_8iz7 TGTCCACACATCCCTCTCTAAGCCCAGGATAG(+CAGTGT)GTACCAATATAGGTAACTCTGCATTTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605110744_apbb CTTATGGGAAAAATGCAGAGTTACCTATATTG(+CAGTGT)GTACCTATCCTGGGCTTAGAGAGGGATGTGT NGG 10 8 17 8 24 0 100 5 10
    240605110743_b5qk TACACCTCTAGGAACTGAGGCAGCTTCAGAAG(+CAGTGT)GTACCTGCCAGATGTTGCTGTGGGAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605110741_2m7n AAGGCCATGCTTCCCACAGCAACATCTGGCAG(+CAGTGT)GTACCTTCTGAAGCTGCCTCAGTTCCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240605110739_0v0c GATGGCTTACAACTGTAACCCTGGTTCCAGGG(+CAGTGT)GATCCAGTACCTTCTGACCTGCTCGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605110738_qcn0 TTCCTGGCACCCGAGCAGGTCAGAAGGTACTG(+CAGTGT)GATCCCCTGGAACCAGGGTTACAGTTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605110736_ytqj TTCCTTGAAGTGAGGTCAAGGTCATGTGGCCG(+CAGTGT)GATCCTATACTCACCACTGGCTGTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605110734_sjt5 CTTGAGAGAAAGACAGCCAGTGGTGAGTATAG(+CAGTGT)GATCCGGCCACATGACCTTGACCTCACTTCA NGG 10 8 17 8 24 0 100 5 10
    240605110733_c2rf TTATCCACAGGACCTGGGTGGAGCAGGATATG(+CAGTGT)GATCCCTCATACTGAAAGATGTAGGGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605110731_h7yz ACTTCCTTTTCCCTACATCTTTCAGTATGAGG(+CAGTGT)GATCCATATCCTGCTCCACCCAGGTCCTGTG NGG 10 8 17 8 24 0 100 5 10