Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110944_nj93 TCTGCTGGCAGGTGGCAAGGCTACGGTGCTGG(+CAGTGT)GTACCCCTAGCCAGCCTTTGACATAGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605110942_pcqj GCTTTTCAACTGACTTCCTCTGGAGTGGGGAG(+CAGTGT)GTACCAACTGATTTCCAGAAGGCTAAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605110941_mtz8 GCGCCGTGCTTTAGCCTTCTGGAAATCAGTTG(+CAGTGT)GTACCTCCCCACTCCAGAGGAAGTCAGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605110939_g1ns TAGAAGCGGGGCTGCCTGCCCAGGAGGACTGG(+CAGTGT)GATCCTCTCTGCTCAGGGAAGCACAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605110938_t8mc GAGGCCTGGCCTGTGCTTCCCTGAGCAGAGAG(+CAGTGT)GATCCCAGTCCTCCTGGGCAGGCAGCCCCGC NGG 10 8 17 8 24 0 100 5 10
    240605110937_2f9b AGGCAAGGGAGGAGCAATCATTTTCCGCAATG(+CAGTGT)GTACCAATGGATGGGGAGGTACTGGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605110935_6iz9 CCCACCCCTCACCAGTACCTCCCCATCCATTG(+CAGTGT)GTACCATTGCGGAAAATGATTGCTCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605110933_dcht AGTGCAATCTGAAATGACCTGGACAAGGAATG(+CAGTGT)GTACCCCATTGGGTTGAAAAATTCACATAGC NGG 10 8 17 8 24 0 100 5 10
    240605110931_mu8e TCCTGGCTATGTGAATTTTTCAACCCAATGGG(+CAGTGT)GTACCATTCCTTGTCCAGGTCATTTCAGATT NGG 10 8 17 8 24 0 100 5 10
    240605110930_t30v CCACAGACCCATTGGGGCCAGGTCTAGCTCAG(+CAGTGT)GTACCTGAGCAAATGTTCCTTTAGAGTCCAC NGG 10 8 17 8 24 0 100 5 10