Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110253_zumd TGCTTCATGCTCAGCAAAGGCTCTGCTGAAAG(+CAGTGT)GTACCCCAGCCCCTGCCAGGTGCTTAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605110251_u6mj CCTGGAGCGCTCTCTCCTGGAGGCAGCGAGGG(+CAGTGT)GTACCACCTCTGCAAATAGACCAGGAAGCGG NGG 10 8 17 8 24 0 100 5 10
    240605110250_taod TGGCACCGCTTCCTGGTCTATTTGCAGAGGTG(+CAGTGT)GTACCCCTCGCTGCCTCCAGGAGAGAGCGCT NGG 10 8 17 8 24 0 100 5 10
    240605110248_tui4 ATGGACTTCCTTGTCTAAAGGCCACCTCGAGG(+CAGTGT)GTACCACCTCAACATGCCAGGGTACTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605110247_uxj4 TGAAGAAAACAGTACCCTGGCATGTTGAGGTG(+CAGTGT)GTACCCTCGAGGTGGCCTTTAGACAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605110245_5567 AGACTGCCAAGTCCGTTCATGTCGCAAGATGG(+CAGTGT)GATCCTGTGGCCCTGAGCCTAATAGTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605110242_g65n GGATGGTGGCACTATTAGGCTCAGGGCCACAG(+CAGTGT)GATCCCATCTTGCGACATGAACGGACTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605110240_znuh GGTGTGTTTTTTTTTTTTTCTTTTTCCCCTTG(+CAGTGT)GTACCTATTGGCATTCTGTAAATCACACTAT NGG 10 8 17 8 24 0 100 5 10
    240605110238_tnxc ACCACATAGTGTGATTTACAGAATGCCAATAG(+CAGTGT)GTACCAAGGGGAAAAAGAAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605110237_5tqo TTACAGCTGACTTAGTACCCGTGAAATGATTG(+CAGTGT)GTACCACTTGAGCATTGTGAATAATTTTTCT NGG 10 8 17 8 24 0 100 5 10