Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110726_v8ai TCTGAACAAAAGGAAGGAAAATGATTGGACAG(+CAGTGT)GTACCCCACAAACTTTCCATCTAAGTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605110724_ohbg GTTGGGCAACACTTAGATGGAAAGTTTGTGGG(+CAGTGT)GTACCTGTCCAATCATTTTCCTTCCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605110722_y6x2 CCCTAGCCTAGGGCTTCGACATTGCATGCTGG(+CAGTGT)GATCCAGCTCCTACCCTTCCTGCACTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605110720_vpas TGGGGAAGGGAGTGCAGGAAGGGTAGGAGCTG(+CAGTGT)GATCCCAGCATGCAATGTCGAAGCCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605110719_jqpk CTAACCACTGCCCTCCTGGAACTGCTGTGCTG(+CAGTGT)GATCCAAAGGCCTCCTCACCAGGAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605110717_axsq GGGCCAGAGCCTTCCTGGTGAGGAGGCCTTTG(+CAGTGT)GATCCAGCACAGCAGTTCCAGGAGGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605110715_zumf ATAGGTGCTGGAGAAGCGGGGACCGCAGTGGG(+CAGTGT)GTACCATCAGGCTCGGGGTAAGGTAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605110714_mx8o TTACCCTTTTCTACCTTACCCCGAGCCTGATG(+CAGTGT)GTACCCCACTGCGGTCCCCGCTTCTCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605110713_euic CTCCTCTACACAGCAGCAACTAAAATGATCAG(+CAGTGT)GATCCTTCACTGGAGCCCTAGAGACTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605110711_75w4 ATCTTCTGGGAGTCTCTAGGGCTCCAGTGAAG(+CAGTGT)GATCCTGATCATTTTAGTTGCTGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10