Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110235_qg7a AATAAAGAAAAATTATTCACAATGCTCAAGTG(+CAGTGT)GTACCAATCATTTCACGGGTACTAAGTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605110234_9a1o CCTCATTATCTACCAAACACATAAGCCAGTGG(+CAGTGT)GATCCCACAAGCGTCCCTTGCCTTGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605110232_0ezr ATATGTGGGGACAAGGCAAGGGACGCTTGTGG(+CAGTGT)GATCCCACTGGCTTATGTGTTTGGTAGATAA NGG 10 8 17 8 24 0 100 5 10
    240605110230_m64i TCCCATTTGTGTTACTGCGACTGAGAAGGCAG(+CAGTGT)GATCCAGGCACCACCTTGAGAGGGAACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605110229_sg2d TGTGGCTCAGTTCCCTCTCAAGGTGGTGCCTG(+CAGTGT)GATCCTGCCTTCTCAGTCGCAGTAACACAAA NGG 10 8 17 8 24 0 100 5 10
    240605110225_k5as ACCACCACAGCAGCCTATCAGCCACTTCTGGG(+CAGTGT)GATCCAGTCCAAGAATGAAAGCCCATAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605110224_r2q4 TCTGCAGCCTATGGGCTTTCATTCTTGGACTG(+CAGTGT)GATCCCCAGAAGTGGCTGATAGGCTGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605110223_ygyj CTGCACAGCACCTCTGAAAGCCACTGGTTGAG(+CAGTGT)GTACCTACCTGTAGGGAGAGGGTGTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240605110221_i55k AGCAAGAAATGACACCCTCTCCCTACAGGTAG(+CAGTGT)GTACCTCAACCAGTGGCTTTCAGAGGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605110220_8uvc ACAATAAATGTTTACATTTCTACTAATTTCTG(+CAGTGT)GATCCACTCACCTGTAAATATGCTGTTCAGC NGG 10 8 17 8 24 0 100 5 10