Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605110029_2c91 GTCCCCCAGTGGCTTTCCCAGCAGCAGAACAG(+CAGTGT)GATCCATACATGTCTATAAGCACTGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605110027_k2o5 CCCATCAGTCTTTAGCCCCAGGCTCAGGCAGG(+CAGTGT)GATCCCTTCATCTGATCCTCTACCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605110026_f98h GTGGGGAGGAGGGGTAGAGGATCAGATGAAGG(+CAGTGT)GATCCCTGCCTGAGCCTGGGGCTAAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605110024_9wjd GCTCTCGCAGAAGATGGAGGTTCAATCTCCAG(+CAGTGT)GATCCAGATGATGGCTCCCAACTGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605110023_tsez TGGAGTTACAGACAGTTGGGAGCCATCATCTG(+CAGTGT)GATCCTGGAGATTGAACCTCCATCTTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240605110021_ss08 ACACACACACACCACACGCAAAGCCATGGGAG(+CAGTGT)GATCCCAGAGGCCTTGTTGAGAATTGGATTC NGG 10 8 17 8 24 0 100 5 10
    240605110019_scb7 CTCATGAATCCAATTCTCAACAAGGCCTCTGG(+CAGTGT)GATCCTCCCATGGCTTTGCGTGTGGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605110017_dlh8 ACAAGGACACTGGGAGGTGTGTGAACGCCATG(+CAGTGT)GTACCGGCGGCTGCTTGAGGCGTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605110014_12gn GAGTCACAGGAAAACGCCTCAAGCAGCCGCCG(+CAGTGT)GTACCATGGCGTTCACACACCTCCCAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605110012_0rv6 GGTGCTCCCAGGGCACTCACCACAGCCACCAG(+CAGTGT)GATCCTCCAGAGGCAAGCAGCATCTCAGATT NGG 10 8 17 8 24 0 100 5 10