Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605105941_6l1p TATTGAGATCTAATGACAATGAATGATATGTG(+CAGTGT)GATCCTGATTTGGGCTCTCAACTGATTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605105940_pbtb GCTGTTAACAGGAGGTCCAAACCTCTTGGAAG(+CAGTGT)GTACCAAGCAGCTAATAAAGGCTGGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605105939_ms4c GATGAAGAAGACCAGCCTTTATTAGCTGCTTG(+CAGTGT)GTACCTTCCAAGAGGTTTGGACCTCCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605105937_gxkr AGTCTCCCTAGCGCCACTGTGCTGAGAACAGG(+CAGTGT)GATCCTCTGGATGAGGAAGCCCTGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605105935_wymx CTGAGCTGGGGCCAGGGCTTCCTCATCCAGAG(+CAGTGT)GATCCCTGTTCTCAGCACAGTGGCGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605105934_6dax CATCTAACAGCAGCTTGAACCTTGGATCTTAG(+CAGTGT)GATCCGCTTGACATAGTCTGCTTTCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240605105932_xqb9 AAGTTCTCCGAGAAAGCAGACTATGTCAAGCG(+CAGTGT)GATCCTAAGATCCAAGGTTCAAGCTGCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605105931_xdh8 AGTGTCTGGGTTTGGTGACTGATTATGGGATG(+CAGTGT)GATCCGGGTGGGGTATGCTCTGGATAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605105929_5vi4 AAGGATGAACTATCCAGAGCATACCCCACCCG(+CAGTGT)GATCCATCCCATAATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605105926_3f3w TCTTTCTCTAGCTCTTCCATTGGGGGACCTGG(+CAGTGT)GATCCATCCAATAGCTGACTGTGAGCATCCA NGG 10 8 17 8 24 0 100 5 10