Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605105837_dibx ACTGCTCCACGGTGTTATTTAGAGCCCCTGGG(+CAGTGT)GTACCGGATTTCTGTTCCTGTCACCTGAACC NGG 10 8 17 8 24 0 100 5 10
    240605105835_mam5 TGTGGCTGGCATTTACATGGATGTGAGGGGAG(+CAGTGT)GATCCGAACTCAAATCTTCACATATGGATGG NGG 10 8 17 8 24 0 100 5 10
    240605105834_kz6c GCTTGCCATCCATATGTGAAGATTTGAGTTCG(+CAGTGT)GATCCTCCCCTCACATCCATGTAAATGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605105832_i37k CTACCGACAGTAAAAGTTAAAAGCAACACCAG(+CAGTGT)GTACCTGCATCCATTTACAATACTTGCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605105830_f0xt CTGTGATTTGCAAGTATTGTAAATGGATGCAG(+CAGTGT)GTACCTGGTGTTGCTTTTAACTTTTACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605105828_i056 CAATTTTACAAATAAATACTTTCTTTCCTTGG(+CAGTGT)GTACCTAGAATAATTAATCCAGCATACAACT NGG 10 8 17 8 24 0 100 5 10
    240605105827_q7is TTCAAAGTTGTATGCTGGATTAATTATTCTAG(+CAGTGT)GTACCCAAGGAAAGAAAGTATTTATTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605105825_ksx5 TAAATGGGCTTCTCAGATAGTACCCTCCACAG(+CAGTGT)GTACCCCCTTAATCAATACCATGCAAATTGT NGG 10 8 17 8 24 0 100 5 10
    240605105823_pi12 TGAGCACAATTTGCATGGTATTGATTAAGGGG(+CAGTGT)GTACCTGTGGAGGGTACTATCTGAGAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605105822_e21c TAAGCCTTCCTTCATTCTGAATACTTCACTTG(+CAGTGT)GTACCATGCTAGGACTGAGAGTCATATTTCA NGG 10 8 17 8 24 0 100 5 10