Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605062642_h51apaq6 ACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTT(/G)TACCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51aojzd TCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTGCACC(/T)CTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGA NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51aod4p CACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTGCACCCC(/T)GCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGATT NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51an2s9 TTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTGCC(/G)CCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51amemc GGGGGAATATAAACAGCCTCTACCCCCATTGCACCCTGCAGTCTCAGATCC(/A)TCGCTCACCCCCTTCTCAGAAATCTCCTCACGATTCCAAGTCACCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51alqxz TTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACC(/T)CCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51alnwm AGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTGCACCCTGCAGG(/C)CTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGATTCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51alcpp ACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCC(/G)CATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATC NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51akd9y CCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCC(/T)ATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCT NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51ajdyw ACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTGCACCCTGCC(/T)GTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGATTCCA NGG 10 8 17 8 24 0 100 5 10