Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605062528_dhmlvz25 CCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCC(/G)GCTATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlv6is GAGATCCGATGAATTTAGGCCTCAAAATTACCACCAGCAGGATTTCCGACC(/G)AATGTCTGATCACCGCCCCGCTATGGGCTACCATGGCCAGGGACCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlupz1 CACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTAA(/G)CATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAAC NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmls6qd AAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCTAA(/G)GGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCACACA NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlnrye CAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCC(/T)ATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCACA NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlndms TCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGG(/A)TATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlmzgs TTTAGGCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCAA(/C)CGCCCCGCTATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCT NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmllfwf GATTTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCATGGCCAA(/T)GGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlj7ao CTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCC(/T)CTATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlfnht GGATTTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCATGGCCC(/G)GGGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAA NGG 10 8 17 8 24 0 100 5 10