Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605062528_dhmlfdry CCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCC(/T)GCTATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmleorq AGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCATT(/G)GCCAGGGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlct5v GGATTTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCATGGCCC(/T)GGGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlb628 TTAGGCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACC(/G)GCCCCGCTATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTC NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhmlaexx AGGCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGG(/A)CCCGCTATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhml7vpv AGGCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGG(/C)CCCGCTATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhml7imf CCGATGAATTTAGGCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGG(/A)CTGATCACCGCCCCGCTATGGGCTACCATGGCCAGGGACCCTCAGACCA NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhml680a AATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCTATT(/C)GGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCACACAG NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhml61i6 GATGAATTTAGGCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCC(/T)GATCACCGCCCCGCTATGGGCTACCATGGCCAGGGACCCTCAGACCATT NGG 10 8 17 8 24 0 100 5 10
    240605062528_dhml534k CCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCC(/T)TGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAACTG NGG 10 8 17 8 24 0 100 5 10