Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605062642_h51aikuy AGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCC(/G)TCTACCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51aijw4 CATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTT(/C)CCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51ai3mj TCAGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAA(/C)CCTCTACCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51afqqo CCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCC(/A)ATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCT NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51ac901 ACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTT(/C)TACCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51aayrz CCTCAGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAAA(/C)AGCCTCTACCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCC NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51aaw3y AAACTGGGGGAATATAAACAGCCTCTACCCCCATTGCACCCTGCAGTCTCC(/T)GATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGATTCCAAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51a99eh TTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTGCAA(/C)CCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACG NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51a7x16 CGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCC(/G)TTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTC NGG 10 8 17 8 24 0 100 5 10
    240605062642_h51a73ti ATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTAA(/G)CCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAA NGG 10 8 17 8 24 0 100 5 10