Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605105957_zvcp AGGTAATCTAGGTAAGATTAGGCCGTTGAGTG(+CAGTGT)GATCCCTCCTAGAGATGTCTGGGCTCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605105955_wan6 TTCTTGGTGGTAAAGGCGCCATGCTGTCTTTG(+CAGTGT)GTACCTGACAGGTTTTAGCAGCCTAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605105954_speu AAAGGTCTTGCTAGGCTGCTAAAACCTGTCAG(+CAGTGT)GTACCAAAGACAGCATGGCGCCTTTACCACC NGG 10 8 17 8 24 0 100 5 10
    240605105952_81rp GTACAAGGGCTTTGCTGATAGTTAGCTGGTGG(+CAGTGT)GTACCACTGACGCTGAGAGCATGCCACCAGA NGG 10 8 17 8 24 0 100 5 10
    240605105951_6c2x AGGGCTCTGGTGGCATGCTCTCAGCGTCAGTG(+CAGTGT)GTACCCACCAGCTAACTATCAGCAAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605105949_bisz TTCCACAGTGGCATGGCCACCCAGCCTCGGGG(+CAGTGT)GATCCTGGGCTCGCTGCACAGCACCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240605105946_f7ao GAAGAGATCTGGGTGCTGTGCAGCGAGCCCAG(+CAGTGT)GATCCCCCGAGGCTGGGTGGCCATGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605105945_ns3i AGCCTCCACACGAGTGTTCTAGTACATGTTAG(+CAGTGT)GATCCTCTCCTGTACCAGTGTCCAGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605105944_uc6o GTCTGTTACACCTGGACACTGGTACAGGAGAG(+CAGTGT)GATCCTAACATGTACTAGAACACTCGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605105943_sopb TTCCACTCAAATCAGTTGAGAGCCCAAATCAG(+CAGTGT)GATCCACATATCATTCATTGTCATTAGATCT NGG 10 8 17 8 24 0 100 5 10