Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031602_x1o3 ATAGGGCCTTCCTGAAGCAGAGGGGTCTGGAG(+ACTCAC)GATCCAGTTGCTGGCCAGCTTCCTCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240605031600_6xjs ATGTCACCTAGGGAGGACCAAGGATAACCAAG(+ACTCAC)GTACCCTTTCTTGGTACTCCCAGGGCCAACC NGG 10 8 17 8 24 0 100 5 10
    240605031559_mkif TCTAGGGTTGGCCCTGGGAGTACCAAGAAAGG(+ACTCAC)GTACCTTGGTTATCCTTGGTCCTCCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605031557_rkhw CTTAGTCAGGGTCATAAGGTACTTTGGTGGTG(+ACTCAC)GATCCTGCTCACTGTTATATAAGTTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605031556_7hft TGAGACCTGCCAACTTATATAACAGTGAGCAG(+ACTCAC)GATCCACCACCAAAGTACCTTATGACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605031553_m5dz TCCAGAGGTTTTTACTGAGAGGACTGAGGGGG(+ACTCAC)GTACCCCAGCTGGAGGGCTGAAGGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605031551_f2iq GCCTTTCCTGGGCCTTCAGCCCTCCAGCTGGG(+ACTCAC)GTACCCCCCTCAGTCCTCTCAGTAAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240605031549_rgq7 CCTTTTAGCTTGGAGGTGGGAGCCATGCCCAG(+ACTCAC)GATCCCTTTCCCGGGCTTTGTCCATGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605031547_1em5 AGGGATTTCCCATGGACAAAGCCCGGGAAAGG(+ACTCAC)GATCCTGGGCATGGCTCCCACCTCCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605031546_a0eb TCTTTCCTCCAGCCCTTTTGGTCTCTTTCCTG(+ACTCAC)GATCCTTGTCCTCCAGACTGGCCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10