Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031529_i0jr AGGGAGACAGTACCCAGGAGAGGAGGCCGCAG(+ACTCAC)GATCCAGCCTGTCTTCAGGATCATCTACACA NGG 10 8 17 8 24 0 100 5 10
    240605031528_h5kd TTGTCTTCCCTGGCTCACAGGGCTCCTGTGAG(+ACTCAC)GATCCAACGATGTGATGGGTCAGGGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605031527_jr7x GTCAACGAGCTCCCTGACCCATCACATCGTTG(+ACTCAC)GATCCTCACAGGAGCCCTGTGAGCCAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605031526_0i2j TCAAGACATGAGTATGAGCACAGTTTCAAATG(+ACTCAC)GTACCAAAATTAATCCATTTCACAGTAATCA NGG 10 8 17 8 24 0 100 5 10
    240605031524_qxz4 AGAAATGATTACTGTGAAATGGATTAATTTTG(+ACTCAC)GTACCATTTGAAACTGTGCTCATACTCATGT NGG 10 8 17 8 24 0 100 5 10
    240605031523_0uhb TGCTAAACTCCAAGCTGAAGGCACAATGAAGG(+ACTCAC)GATCCTTGGTACCTTGAGCATTTCCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605031521_y6b3 CCTTTTTCAAAGGAAATGCTCAAGGTACCAAG(+ACTCAC)GATCCCTTCATTGTGCCTTCAGCTTGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605031520_e2nf TACCAAAAGGTCTGTCTGACCAGAGACTGAAG(+ACTCAC)GTACCAAGGGAACTGGGCTTTTTTTCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240605031517_kb2a CCCTGCGCCTGAAAAAAAGCCCAGTTCCCTTG(+ACTCAC)GTACCTTCAGTCTCTGGTCAGACAGACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605031516_scvi TGGCCTTTGGTCCCAGACGGTCCAGGTCTGAG(+ACTCAC)GATCCAATAGGGAAGCTGGAGCACTGGGAAA NGG 10 8 17 8 24 0 100 5 10