Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031908_ro3p TAAGGAAAAGGAATAAGCAGGGACAGCTTTGG(+ACTCAC)GATCCTGGGTCGGAGTAGTCTGTCCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605031906_0l01 TTCAAGCCTAAAGACCTGAGTTTAGTCCTTAG(+ACTCAC)GATCCACAAGCTGGAAAAAGAAAACCAACTC NGG 10 8 17 8 24 0 100 5 10
    240605031905_iiew TTGTGGAGTTGGTTTTCTTTTTCCAGCTTGTG(+ACTCAC)GATCCTAAGGACTAAACTCAGGTCTTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605031904_iq31 CTGGAAGGTCTCATGCAATCCAAGCTGACTTG(+ACTCAC)GATCCTCTTGTCTCAGCCTCTTGAGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605031902_wv1u TGTCCTAGCTACTCAAGAGGCTGAGACAAGAG(+ACTCAC)GATCCAAGTCAGCTTGGATTGCATGAGACCT NGG 10 8 17 8 24 0 100 5 10
    240605031900_xf5u CTGCTAATCCCCTTCATCTTCCCAGTTGGTGG(+ACTCAC)GATCCAGTCAATTGCATTAGAATTGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240605031859_t0ff TACTCCAGTAACAATTCTAATGCAATTGACTG(+ACTCAC)GATCCCACCAACTGGGAAGATGAAGGGGATT NGG 10 8 17 8 24 0 100 5 10
    240605031857_su4n TCCCTCCCCACCGACATGACTCCCAAAGTGGG(+ACTCAC)GTACCAGACAGCATGGGGGTTAAGATTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605031856_8moh TAGGAAACTAATCTTAACCCCCATGCTGTCTG(+ACTCAC)GTACCCCACTTTGGGAGTCATGTCGGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605031853_artg GCTGGTTCCAGCTGCTGTTCCATGGGCCCTTG(+ACTCAC)GTACCAAGGACTCGTATGTGTCCCCATTTGA NGG 10 8 17 8 24 0 100 5 10