Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031430_sl59 GCGCGCAGCCTGATACTGCTTTTTCTCCCGTG(+ACTCAC)GATCCAGAATTCCTAGAGGCATCACCGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605031428_nkfs TGCACAAGAGATGAGGAATATCAGAGAGATGG(+ACTCAC)GATCCAATTCGATATTTAAACCTTTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605031427_qc5j AGAAACCAGATAAAGGTTTAAATATCGAATTG(+ACTCAC)GATCCCATCTCTCTGATATTCCTCATCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605031426_vun2 GTCCTCTCCTTCCAGCTTTACATGGTTCCAAG(+ACTCAC)GATCCGATGGTCAGGTTTGCATTATCATGTG NGG 10 8 17 8 24 0 100 5 10
    240605031424_0ta0 TGAAGCACATGATAATGCAAACCTGACCATCG(+ACTCAC)GATCCTTGGAACCATGTAAAGCTGGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605031422_qmrw GGATTCTCAGGAGCTTCTCTCCCTACCCAATG(+ACTCAC)GATCCACCCCCATCACACACATTTACATACA NGG 10 8 17 8 24 0 100 5 10
    240605031421_juqp GGGCTTGTATGTAAATGTGTGTGATGGGGGTG(+ACTCAC)GATCCATTGGGTAGGGAGAGAAGCTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605031420_1omt AACTGAAGTGATACAGTTCAAATGCCAGCGAG(+ACTCAC)GATCCATGCTTGAAGACAAGTATTAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240605031418_nyej ATCATTTATCCTAATACTTGTCTTCAAGCATG(+ACTCAC)GATCCTCGCTGGCATTTGAACTGTATCACTT NGG 10 8 17 8 24 0 100 5 10
    240605031417_vod8 CTGCCCTTAAATGATCTGGACTCACCTGGGAG(+ACTCAC)GTACCTGGGCTGCCCAAGGCCCATGGACATT NGG 10 8 17 8 24 0 100 5 10