Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031415_ai72 GAATCAATGTCCATGGGCCTTGGGCAGCCCAG(+ACTCAC)GTACCTCCCAGGTGAGTCCAGATCATTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605031413_38ww CTGCCCTTAACTGATCCGGACTCACCTGGGAG(+ACTCAC)GTACCTGGGCTGCCCACGGCCCATGGACATT NGG 10 8 17 8 24 0 100 5 10
    240605031410_5f04 GAATCAATGTCCATGGGCCGTGGGCAGCCCAG(+ACTCAC)GTACCTCCCAGGTGAGTCCGGATCAGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605031408_j0ps GAATCAATGTCCATGGGCCGTGGGCAGCCCAG(+ACTCAC)GTACCTCCCAGGTGAGTCCGGGTCAGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605031406_64fm CTGCCCTTAACTGACCCGGACTCACCTGGGAG(+ACTCAC)GTACCTGGGCTGCCCACGGCCCATGGACATT NGG 10 8 17 8 24 0 100 5 10
    240605031405_uo33 AAGTTGATAGAAATTGTCTTATCCATATTCTG(+ACTCAC)GTACCTGAGTAATTCTGCCATTTATTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605031403_0435 CCATGCATCTAATAAATGGCAGAATTACTCAG(+ACTCAC)GTACCAGAATATGGATAAGACAATTTCTATC NGG 10 8 17 8 24 0 100 5 10
    240605031401_3ugd CCCAGTTAAGTATCTCACAACTGACTCCAAGG(+ACTCAC)GATCCAACAGAATTCTGGCATCTGAGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605031400_vmk8 TGCAGGTGCACTCAGATGCCAGAATTCTGTTG(+ACTCAC)GATCCCTTGGAGTCAGTTGTGAGATACTTAA NGG 10 8 17 8 24 0 100 5 10
    240605031359_zf7t TTTCCAAATTGTATACAAGTCGATTCATTAAG(+ACTCAC)GATCCAACTAGTAACATCATTCATTTAATGC NGG 10 8 17 8 24 0 100 5 10