Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031358_d6i1 ACAAAGCATTAAATGAATGATGTTACTAGTTG(+ACTCAC)GATCCTTAATGAATCGACTTGTATACAATTT NGG 10 8 17 8 24 0 100 5 10
    240605031356_wien GATCAGTTGAGAGCCTTCTGATAACACTGGAG(+ACTCAC)GATCCTGGTCTACAGATGTAGCCAATCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605031355_vrsv AAGCATCCAGATTGGCTACATCTGTAGACCAG(+ACTCAC)GATCCTCCAGTGTTATCAGAAGGCTCTCAAC NGG 10 8 17 8 24 0 100 5 10
    240605031353_80jp AGAACCAGTGAGTTAAGTAGCCTGGGAACTGG(+ACTCAC)GTACCATTTGAGTCAAGCCTTGAAACATGGG NGG 10 8 17 8 24 0 100 5 10
    240605031352_sru4 CCACACCCATGTTTCAAGGCTTGACTCAAATG(+ACTCAC)GTACCCAGTTCCCAGGCTACTTAACTCACTG NGG 10 8 17 8 24 0 100 5 10
    240605031350_0i37 GTTCTCCCAAGTAGAGTAAATGGTTCATATGG(+ACTCAC)GTACCAAACACCAGGAAGCAAAATGAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605031349_efdd CTTTGTTCTTTCATTTTGCTTCCTGGTGTTTG(+ACTCAC)GTACCCATATGAACCATTTACTCTACTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605031348_3u6s TCCTTTTCGCTTAATGAAAGAGGTTGCTTCAG(+ACTCAC)GATCCATTGCTGCAGAGAATAATAATTTATT NGG 10 8 17 8 24 0 100 5 10
    240605031347_3y46 ACAGCAATAAATTATTATTCTCTGCAGCAATG(+ACTCAC)GATCCTGAAGCAACCTCTTTCATTAAGCGAA NGG 10 8 17 8 24 0 100 5 10
    240605031346_58eo CAGTATTCATTCCATGAACATAAGAAATAATG(+ACTCAC)GTACCTAAAATTAAAAAACAAAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10