Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031500_1kvm AAGTGAAAATCGTAGGTAGTGCTTCACCTTAG(+ACTCAC)GATCCTTGGAAAGATTCTTGATGTTTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605031458_qni5 GTAGAGTTTTTATAAGGCCAGTGAGTTAACTG(+ACTCAC)GTACCCTGTACTGCCAAATCTAATTTTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605031457_b94x CTAACCCTAAAAATTAGATTTGGCAGTACAGG(+ACTCAC)GTACCAGTTAACTCACTGGCCTTATAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605031455_230l CTAACCCTAAAAATTAGATTTGGCAGTACAGG(+ACTCAC)GTACCAGTTAACTCACTGGCCTTATAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605031454_uhv8 GTAGAGTTTTTATAAGGCCAGTGAGTTAACTG(+ACTCAC)GTACCCTGTACTGCCAAATCTAATTTTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605031453_f8og GTCAATTTAATGCTTTAAAAGTAACTTATCAG(+ACTCAC)GATCCACTTCAATCTCCACATGATAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240605031452_lvl4 TAATCCTTTATTATCATGTGGAGATTGAAGTG(+ACTCAC)GATCCTGATAAGTTACTTTTAAAGCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605031451_0q12 TTGTGCTTTGTAAAGCAAACTCGAGAAAACTG(+ACTCAC)GTACCAGTGTCTGTACCTCAGCTGGGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605031449_nczu AGATGAAGGACCCAGCTGAGGTACAGACACTG(+ACTCAC)GTACCAGTTTTCTCGAGTTTGCTTTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605031448_oqhe ATTCAAAGTTAGAGTGAATCCATGCCAAAAGG(+ACTCAC)GATCCCTCACACTGGATAAGAGAAACCAGTG NGG 10 8 17 8 24 0 100 5 10