Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025412_atgu GACCCCAGGTTTCACAAATGCTAGAGATGTAG(+ACTCAC)GATCCCTTGGGAAAAGGGGTGTTTTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605025411_0z7m ACTCCCCCATAGGGTCAAACGGCTCAACCAAG(+ACTCAC)GTACCTGGCCAGGACACAGTCCTCATGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605025409_u805 AGGAGCTAGCATGAGGACTGTGTCCTGGCCAG(+ACTCAC)GTACCTTGGTTGAGCCGTTTGACCCTATGGG NGG 10 8 17 8 24 0 100 5 10
    240605025408_x2sl CGGTCTGGAGCCAGCGGAGAAGGAGGAGATTG(+ACTCAC)GATCCCTCAGAAGGGCTCCCCGTGCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605025406_e796 GGGGTGGGGCTGCACGGGGAGCCCTTCTGAGG(+ACTCAC)GATCCAATCTCCTCCTTCTCCGCTGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605025405_jfqo AGATTTTGAGATCACATCGTTCTCCCCTCAAG(+ACTCAC)GATCCCCTGATTAGTGGGAGACTAAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240605025403_ygqb GGGGGCAGGTCTTAGTCTCCCACTAATCAGGG(+ACTCAC)GATCCTTGAGGGGAGAACGATGTGATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605025402_62hx TGAGAGAAGGGCTGGGAGCCAGCCAAGGGATG(+ACTCAC)GTACCTTTGTTGTGACGGGGCTACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605025400_rh84 GCTGAGAAGGGTGTAGCCCCGTCACAACAAAG(+ACTCAC)GTACCATCCCTTGGCTGGCTCCCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605025359_khs8 AGAAGTCCAGATACTCCATTCTTGAGTGTCAG(+ACTCAC)GATCCCAGGCAAAACTGGCAATACAATCCAG NGG 10 8 17 8 24 0 100 5 10