Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025513_jsjp AAACATATACAAACCTTGTCACTTAAAATGTG(+ACTCAC)GTACCAGGAAGACAAGCTCTGCTGATATTTT NGG 10 8 17 8 24 0 100 5 10
    240605025512_de6g GATGAGAAGGGTAAGTCTTCCCAGAGGAAGTG(+ACTCAC)GTACCAATACCTGTATGCTGGGCATCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605025510_oppe TCTTCCTCAGGATGCCCAGCATACAGGTATTG(+ACTCAC)GTACCACTTCCTCTGGGAAGACTTACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605025509_mv12 TGTGTACTATGGAGATGCATATTCTTTCCAAG(+ACTCAC)GTACCTTTCTCACACGAATTTCACAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605025507_esk3 GCTCTGAGATCTGTGAAATTCGTGTGAGAAAG(+ACTCAC)GTACCTTGGAAAGAATATGCATCTCCATAGT NGG 10 8 17 8 24 0 100 5 10
    240605025506_okwd CACCTGCAGTAATGTCCTAACTGTGCATTCTG(+ACTCAC)GATCCTCCAAGAGGACGAGCAAGGGAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605025505_n6np GTGTGCTCACTCCCTTGCTCGTCCTCTTGGAG(+ACTCAC)GATCCAGAATGCACAGTTAGGACATTACTGC NGG 10 8 17 8 24 0 100 5 10
    240605025504_2l3u TTGTTGTGCGTGCATGCTAGGAGCTACATTAG(+ACTCAC)GTACCTGGGAATGAGTCAATCTTGTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605025501_ie9d GTATCTATTTTACAAGATTGACTCATTCCCAG(+ACTCAC)GTACCTAATGTAGCTCCTAGCATGCACGCAC NGG 10 8 17 8 24 0 100 5 10
    240605025459_jbej AACATAATACATTTTTATTCCATAGCTTAAGG(+ACTCAC)GATCCATCAGATAAAATGTTGGTCTTTCATC NGG 10 8 17 8 24 0 100 5 10