Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025544_b4md CTGTATATGTAGGTGCCCATGGAGGGATGTTG(+ACTCAC)GATCCCCTGGAGCTAGAGTTTCATGAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605025542_k2y9 CAACTTACACTCATGAAACTCTAGCTCCAGGG(+ACTCAC)GATCCAACATCCCTCCATGGGCACCTACATA NGG 10 8 17 8 24 0 100 5 10
    240605025539_xy3v GCCTAGTGCTACAGAGCCAGGCAGGAACCAGG(+ACTCAC)GTACCAGCCTCTAGGCCTGGTGCCTGCATCA NGG 10 8 17 8 24 0 100 5 10
    240605025538_lagw AGAAGTGATGCAGGCACCAGGCCTAGAGGCTG(+ACTCAC)GTACCCTGGTTCCTGCCTGGCTCTGTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605025536_bb4p ATGAAGTTGTGAGCAGCTGCTCTGTCTTTTGG(+ACTCAC)GATCCTGTTACGATCTGACAAATTAAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605025535_02iu TAAGGCAGACTTAATTTGTCAGATCGTAACAG(+ACTCAC)GATCCCAAAAGACAGAGCAGCTGCTCACAAC NGG 10 8 17 8 24 0 100 5 10
    240605025534_gght GTTGACCTGGTGATATTATCCTGGAGTCTCTG(+ACTCAC)GATCCAGCCACGCTTCATCTCCAACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240605025532_3jgy GGAAAGGATGAGTTGGAGATGAAGCGTGGCTG(+ACTCAC)GATCCAGAGACTCCAGGATAATATCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240605025531_23th GAGCTACAAACCATGTTTGTAAATGCTCAGCG(+ACTCAC)GATCCTTTCAACACACTCTAAGATACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605025530_fhfw TGTTCTTTGAGTATCTTAGAGTGTGTTGAAAG(+ACTCAC)GATCCGCTGAGCATTTACAAACATGGTTTGT NGG 10 8 17 8 24 0 100 5 10