Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025444_7djp GGAGAGCAAGATACTCCCCGGGCCTCGGTGAG(+ACTCAC)GATCCAGGCACCCAGACTCATGGCTTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605025442_np9i AAATAAAATAAATAGAATAAAGTATTCAAAGG(+ACTCAC)GTACCACCACTCCAATTGCATCAAGCCACCT NGG 10 8 17 8 24 0 100 5 10
    240605025440_q3n8 GTCCAAGGTGGCTTGATGCAATTGGAGTGGTG(+ACTCAC)GTACCCTTTGAATACTTTATTCTATTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605025439_dvyd AAGTATTGTTTCCTAATCAGACTTTTACATTG(+ACTCAC)GTACCATGAGTTCTTTATGCTTTGCCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605025438_ba0w ACAATGTCTTGGCAAAGCATAAAGAACTCATG(+ACTCAC)GTACCAATGTAAAAGTCTGATTAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605025436_atyz ACAACCTTGCGAGCAACTTGAGCTTCCTCCAG(+ACTCAC)GATCCTGCTTGATTAGACCCTCAGAGATAGC NGG 10 8 17 8 24 0 100 5 10
    240605025435_d0wn CAATGGCTATCTCTGAGGGTCTAATCAAGCAG(+ACTCAC)GATCCTGGAGGAAGCTCAAGTTGCTCGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605025433_45h6 TATCCCGAGGACAGTTACTAAGCTATACTTGG(+ACTCAC)GATCCCACTCAGAAGGTTTCAGAACTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605025431_niks GAAGGACCCCAGTTCTGAAACCTTCTGAGTGG(+ACTCAC)GATCCCAAGTATAGCTTAGTAACTGTCCTCG NGG 10 8 17 8 24 0 100 5 10
    240605025429_sbz0 CCATCCACACCCTGCAGCAAGTCCCAACCTGG(+ACTCAC)GTACCACTGTTATTATTACAAGGAAAGAAAG NGG 10 8 17 8 24 0 100 5 10