Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025649_akww GGTCACAGGGACAGCTCCGCTGCTGCCTCTGG(+ACTCAC)GTACCTTGAAAAGATGCCCACCACGCACCTT NGG 10 8 17 8 24 0 100 5 10
    240605025648_6av3 AAAGAAAGGTGCGTGGTGGGCATCTTTTCAAG(+ACTCAC)GTACCCAGAGGCAGCAGCGGAGCTGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605025645_vl27 CTGCTGCCCCTGGGTGCAGGCAGTAGCCAGGG(+ACTCAC)GATCCAGGGCCTCAGATCCAGGGTCCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605025643_gebk GTCCGATGCAGGACCCTGGATCTGAGGCCCTG(+ACTCAC)GATCCCCTGGCTACTGCCTGCACCCAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605025642_oxlv CTGCCGGGGAGATGGGCAGGGTGCACTCTGAG(+ACTCAC)GTACCCGCTCCTACAGTCCCAGAACACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605025640_twjr AAGAGAGGGGTGTTCTGGGACTGTAGGAGCGG(+ACTCAC)GTACCTCAGAGTGCACCCTGCCCATCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605025638_vjph CCAGCCACGCACAAGGGGTGGGAGTGGAGTGG(+ACTCAC)GATCCTTAGACTGAAATGCGGTGACACATGG NGG 10 8 17 8 24 0 100 5 10
    240605025637_cqde GAGGACCATGTGTCACCGCATTTCAGTCTAAG(+ACTCAC)GATCCCACTCCACTCCCACCCCTTGTGCGTG NGG 10 8 17 8 24 0 100 5 10
    240605025636_kgga GCCTTTGCAGAGAAATGTTAATCCTGCGTGAG(+ACTCAC)GTACCAGCTGCTCCCAGGACCCTCCGAGCGA NGG 10 8 17 8 24 0 100 5 10
    240605025635_vsef TACTCTCGCTCGGAGGGTCCTGGGAGCAGCTG(+ACTCAC)GTACCTCACGCAGGATTAACATTTCTCTGCA NGG 10 8 17 8 24 0 100 5 10