Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025724_sf4h CCCAGGTCCGCTCTGAACACGTGGACCAAAGG(+ACTCAC)GATCCAGGCAGCTGTGTACATTAAGATGGGT NGG 10 8 17 8 24 0 100 5 10
    240605025722_mwd0 TCCCCACCCATCTTAATGTACACAGCTGCCTG(+ACTCAC)GATCCCTTTGGTCCACGTGTTCAGAGCGGAC NGG 10 8 17 8 24 0 100 5 10
    240605025720_8bc2 CTGGGGTGAAGGGATAGCCCTCCCCCTGCTGG(+ACTCAC)GATCCCCCCAGCCCCTCCGGTCTGGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605025718_xb97 CCCCTTTCCTGCCAGACCGGAGGGGCTGGGGG(+ACTCAC)GATCCCAGCAGGGGGAGGGCTATCCCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605025716_2939 CTCCTGTTCCAAATGGTCTCCTTTAGCCTCTG(+ACTCAC)GATCCAACATGACAACCCAGACTAGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605025715_s3pl GGCTTGAAGCTCTAGTCTGGGTTGTCATGTTG(+ACTCAC)GATCCAGAGGCTAAAGGAGACCATTTGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605025713_zhka CCAGCTCTAGAAAAACAGTGAGCAGCTTTGGG(+ACTCAC)GATCCCCTCCAAGCTGGCTCTACATCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605025711_li0q ATTATGCTTTGATGTAGAGCCAGCTTGGAGGG(+ACTCAC)GATCCCCAAAGCTGCTCACTGTTTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605025708_hy1n CCAGCTCTAGAAAAACAGTGAGCAGCTTTGGG(+ACTCAC)GATCCCCTCCAAGCTGGCTCTACATCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605025706_bv2f ATTATGCTTTGATGTAGAGCCAGCTTGGAGGG(+ACTCAC)GATCCCCAAAGCTGCTCACTGTTTTTCTAGA NGG 10 8 17 8 24 0 100 5 10