Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025705_yqoq GCCTTCTTGGGACAAGGAAGAGAAGAAGGAAG(+ACTCAC)GATCCAAGCAAAAGGTGGTCTCTCCTGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605025704_089n GGGGCCGAGCAGGAGAGACCACCTTTTGCTTG(+ACTCAC)GATCCTTCCTTCTTCTCTTCCTTGTCCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605025702_uu7i ACTCTGTAGAACAGTCTGGCCTTGAACTCAAG(+ACTCAC)GATCCACCTGCTTCTGCCTCTTGGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605025701_ova9 TAATACCAGCACCCAAGAGGCAGAAGCAGGTG(+ACTCAC)GATCCTTGAGTTCAAGGCCAGACTGTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605025659_eouw TGGTGTGATGCCTTCTTGTAGTGTTCTCTGGG(+ACTCAC)GATCCCTCATACCCCAGCCACAGCACAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605025657_orud TCTGGAAACTGTGCTGTGGCTGGGGTATGAGG(+ACTCAC)GATCCCCAGAGAACACTACAAGAAGGCATCA NGG 10 8 17 8 24 0 100 5 10
    240605025656_kww8 TACCCCTCCTGTGTGTGAAGGCTCTGCTGAAG(+ACTCAC)GTACCTTTGGGTCTGGTCTGACCTTACTGCT NGG 10 8 17 8 24 0 100 5 10
    240605025654_nw56 GACAAAGCAGTAAGGTCAGACCAGACCCAAAG(+ACTCAC)GTACCTTCAGCAGAGCCTTCACACACAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605025652_jwsg CGGGCCACCACCTGCCTCTCTCTTATCCAGTG(+ACTCAC)GTACCGCCCAGGCCTTAAATCACCCCGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605025650_qhms CACTGGGAACGGGGTGATTTAAGGCCTGGGCG(+ACTCAC)GTACCACTGGATAAGAGAGAGGCAGGTGGTG NGG 10 8 17 8 24 0 100 5 10