Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030234_yyrr ACCAGTCACAAACCTGTGCTCAAAGCAAACAG(+ACTCAC)GATCCAAACTAAAGGCAGAGCTGTGCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605030232_v6y9 CGCTCCGGTCTAGGAACCTGTCAAGACAGTGG(+ACTCAC)GATCCCAGAATTCCCAAAGTTGCCCAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605030231_418c GTTGCCCCTCTGGGCAACTTTGGGAATTCTGG(+ACTCAC)GATCCCACTGTCTTGACAGGTTCCTAGACCG NGG 10 8 17 8 24 0 100 5 10
    240605030230_tsk3 CCTGTCCTTCCCTTGATTACTGTTGCTTCTTG(+ACTCAC)GATCCTAGATCCCTGGTGAGTCGAGGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605030228_0v4n CCTTTGAGATCCTCGACTCACCAGGGATCTAG(+ACTCAC)GATCCAAGAAGCAACAGTAATCAAGGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605030226_9s3k CCCTTGCTAAAGACTCGGAGAAGACAGCCGAG(+ACTCAC)GTACCAGTGGCAAAGTGTGCCCATGCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605030224_wlu0 ACACGAAAGAGCATGGGCACACTTTGCCACTG(+ACTCAC)GTACCTCGGCTGTCTTCTCCGAGTCTTTAGC NGG 10 8 17 8 24 0 100 5 10
    240605030223_nzqx ACACTGCTTTTCTGGGTCACCTAAGTAGGAGG(+ACTCAC)GATCCATGGAGAACCTGTGTCATCAGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605030221_e4qz TAAGAAGGCCCTGATGACACAGGTTCTCCATG(+ACTCAC)GATCCCTCCTACTTAGGTGACCCAGAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605030219_8byj CCTATGTAGTAACTGATTTCTGCACACTGGAG(+ACTCAC)GTACCTGTTTGCCTTTAAAAGGTATGATAAA NGG 10 8 17 8 24 0 100 5 10