Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025633_qwfu CACATCTCAGAAACAATATCTTGTTGCCAATG(+ACTCAC)GTACCCACACATTACATGTCCATGCATACAC NGG 10 8 17 8 24 0 100 5 10
    240605025631_9zo5 GCATGGTGTATGCATGGACATGTAATGTGTGG(+ACTCAC)GTACCATTGGCAACAAGATATTGTTTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605025630_nfc4 GCTTACTTTCTACAAGCTAACACGGTCTACTG(+ACTCAC)GATCCCGCTTATTTCAAGGTGGGCTTTCATT NGG 10 8 17 8 24 0 100 5 10
    240605025629_ushn TCACCAATGAAAGCCCACCTTGAAATAAGCGG(+ACTCAC)GATCCAGTAGACCGTGTTAGCTTGTAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605025627_w5cg TCTCTAGAGCACGTCTGGACTCTCCTTGCCTG(+ACTCAC)GATCCCACCACACCCTTCCAGGGCCAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605025625_bti9 CTGCCACCTCTGGCCCTGGAAGGGTGTGGTGG(+ACTCAC)GATCCAGGCAAGGAGAGTCCAGACGTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605025623_schh TGGTTGACATACATGGGAATACCAAGTTTGGG(+ACTCAC)GATCCTTTTATTTAGAATATCAAGAAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605025622_0qp4 GAGATTCTTTTTCTTGATATTCTAAATAAAAG(+ACTCAC)GATCCCCAAACTTGGTATTCCCATGTATGTC NGG 10 8 17 8 24 0 100 5 10
    240605025621_544t TCACTGTGCTGTCCATTACTAGCTCCCAAATG(+ACTCAC)GTACCCTTCACCTTAACCATCACAGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605025619_rr1q CTACTACACTGCTGTGATGGTTAAGGTGAAGG(+ACTCAC)GTACCATTTGGGAGCTAGTAATGGACAGCAC NGG 10 8 17 8 24 0 100 5 10