Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025301_rw6a TTAGAGAAAGTGATTATTACAAATCCTTCAAG(+ACTCAC)GATCCCAAAGACTTTCAAATCCATTTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605025259_vyoh GGCCCCGGTAATTAAGAGGTGAAATTCGCCTG(+ACTCAC)GTACCAAGTGGGGCTGTGAACTTAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605025258_9v3h AATTATTTAATTTAAGTTCACAGCCCCACTTG(+ACTCAC)GTACCAGGCGAATTTCACCTCTTAATTACCG NGG 10 8 17 8 24 0 100 5 10
    240605025255_4g37 TTTCAAGTATCGTGATGGATGAAGATGGGGGG(+ACTCAC)GATCCTTCGCTTACATCTCCACACTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605025254_0jy7 CTGAAAACCAAAGTGTGGAGATGTAAGCGAAG(+ACTCAC)GATCCCCCCCATCTTCATCCATCACGATACT NGG 10 8 17 8 24 0 100 5 10
    240605025253_gdbr TTGAGCTCAAGTGAATTGACTTTTTTTTGACG(+ACTCAC)GATCCAGAAGACCACATGAGATTTCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605025252_rfy7 TTTACCTCCTGGAAATCTCATGTGGTCTTCTG(+ACTCAC)GATCCGTCAAAAAAAAGTCAATTCACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605025250_nwuk GACACTCATATCCATTGGCACTGACAGCCTTG(+ACTCAC)GTACCAGAAGGTTAGGTGAATGAACGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605025249_xx3j AGGAAGACCCCGTTCATTCACCTAACCTTCTG(+ACTCAC)GTACCAAGGCTGTCAGTGCCAATGGATATGA NGG 10 8 17 8 24 0 100 5 10
    240605025247_t9bd ATGACAATGCAAGGTTCACCTCAGTGTTGAGG(+ACTCAC)GATCCAAGCTGAGGTGAAGCTGGGGACTATG NGG 10 8 17 8 24 0 100 5 10