Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025157_orkh TAGCTTCGTCCTCATTCCCTATGCGCACCGAG(+ACTCAC)GTACCCGCCCCATAGAAAGCTCTAGAACCTG NGG 10 8 17 8 24 0 100 5 10
    240605025155_xuhc TCATGTTTCTCACTGGGGTCTTGGAACCTGTG(+ACTCAC)GATCCCAGTCCCTATGTGATATGCTCAAATG NGG 10 8 17 8 24 0 100 5 10
    240605025153_5rwf CAGGGCATTTGAGCATATCACATAGGGACTGG(+ACTCAC)GATCCACAGGTTCCAAGACCCCAGTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605025152_6zy9 CATTCTACCCCATTATTTTAGTATTGGTATTG(+ACTCAC)GATCCAATCAGGGCAAGAACATTGTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240605025151_m8wt TTTAATAACATACAATGTTCTTGCCCTGATTG(+ACTCAC)GATCCAATACCAATACTAAAATAATGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240605025149_yq45 GCTTCCCTCAACCTCTCCCTGGCCTGGAGGAG(+ACTCAC)GATCCCAGAGGAAAGGGTTTTCTATGACTGA NGG 10 8 17 8 24 0 100 5 10
    240605025148_q1yc CTAGCTCAGTCATAGAAAACCCTTTCCTCTGG(+ACTCAC)GATCCTCCTCCAGGCCAGGGAGAGGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605025146_mafc ACACGTGCTGTTATCTAACAAGGAAAAGTTTG(+ACTCAC)GATCCTGAGAAAGCAAATAAACATTTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240605025145_tcro AAAATACTTGAAATGTTTATTTGCTTTCTCAG(+ACTCAC)GATCCAAACTTTTCCTTGTTAGATAACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605025144_i9q1 ACAGGAAGTTCCTTAAAAGCATATCATCTTAG(+ACTCAC)GTACCCTCTCTGAGGGTATGACTTCAAATTA NGG 10 8 17 8 24 0 100 5 10