Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025110_17ka TGAAGTATAGTGCAAGAAAGAAATCTAAAATG(+ACTCAC)GTACCAGTACTGCTGTGAGTTCATCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605025109_9l58 TCTGGGGATGTGGGATCTGCTTCTAAGTGTGG(+ACTCAC)GTACCATGTCTACTCTCACTTTATAAAATTA NGG 10 8 17 8 24 0 100 5 10
    240605025107_jcnl AAGGTTAATTTTATAAAGTGAGAGTAGACATG(+ACTCAC)GTACCCACACTTAGAAGCAGATCCCACATCC NGG 10 8 17 8 24 0 100 5 10
    240605025105_pio0 CCTGTAGCAGGCTCTGCTTTTCGGGTCCCATG(+ACTCAC)GTACCAGATTCTTGAAATAAGGTCATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605025104_43cu CTAACTCTTCATGACCTTATTTCAAGAATCTG(+ACTCAC)GTACCATGGGACCCGAAAAGCAGAGCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605025102_1mlk GCTGATGATATGTACGTGCCTGAAACTGGATG(+ACTCAC)GTACCTTTAACTGACACTTAGAAAGCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605025101_r1m8 GCTAATAAAAGCTTTCTAAGTGTCAGTTAAAG(+ACTCAC)GTACCATCCAGTTTCAGGCACGTACATATCA NGG 10 8 17 8 24 0 100 5 10
    240605025100_rnhg CAGTGCACTGCACCTCGGAACACATGCAGTTG(+ACTCAC)GATCCTAAGGATGTTTAAAAGCGATTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605025058_zcm8 GAGACTTTAAAATCGCTTTTAAACATCCTTAG(+ACTCAC)GATCCAACTGCATGTGTTCCGAGGTGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605025056_xm2u ACAGCCGCATTCCAGGGTGGGAGAGCGTGCTG(+ACTCAC)GTACCATCTTCTCTCAAGTAGCTCTGTCATC NGG 10 8 17 8 24 0 100 5 10