Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025027_d7uh GTTTAATTAAACATATAAAAACATCATGGCTG(+ACTCAC)GATCCAGAGACCACAGCCTCAGACTCATTGG NGG 10 8 17 8 24 0 100 5 10
    240605025025_i6if AGTTGTGAAAGCAAGGAGTACAGAATGCTAGG(+ACTCAC)GATCCTCTCAAAAGTTCCAAACTAATTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605025024_9bm7 GGAACACAGAATTAGTTTGGAACTTTTGAGAG(+ACTCAC)GATCCCTAGCATTCTGTACTCCTTGCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605025023_g569 TTGCCCAGGCCCCTCCAGAAGTACTGTGTTTG(+ACTCAC)GTACCAGCATTTGTTTGTAGTTTTTATATGT NGG 10 8 17 8 24 0 100 5 10
    240605025021_q5li CAATCACATATAAAAACTACAAACAAATGCTG(+ACTCAC)GTACCAAACACAGTACTTCTGGAGGGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605025020_emqn ATCTATGAACATGTTTGGTTGGGATGTGTTTG(+ACTCAC)GATCCAGGACCTGGGTCCCCTGGGAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240605025019_oh84 GCCCTAACATTTCCCAGGGGACCCAGGTCCTG(+ACTCAC)GATCCAAACACATCCCAACCAAACATGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605025017_e4tu TAGACTCCCTCTGGGAGCTCTTGGTGGCTTGG(+ACTCAC)GTACCCTGACCTGGCCTTGGGGACTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605025015_1dzn CAGTGCTCAGGAGTCCCCAAGGCCAGGTCAGG(+ACTCAC)GTACCCAAGCCACCAAGAGCTCCCAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605025013_qq9i TTTTGGAACTGTTGCGCTGTGCCCTGGGGGGG(+ACTCAC)GATCCCCGCTCAGACTGGCCTCCCACACCCA NGG 10 8 17 8 24 0 100 5 10