Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025126_vp3q CGGCTGTCCAACATCCTCTGCACTGTAAGAGG(+ACTCAC)GATCCCTTCTTTTGAGACAGTACACATTTTC NGG 10 8 17 8 24 0 100 5 10
    240605025125_a2zu GGTCCCGTCCTGGCAGGGGTTGAAGGGGAGAG(+ACTCAC)GTACCCCCACATGCTGGGGACCTCAGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605025122_h954 TGAAACTGTGCTGAGGTCCCCAGCATGTGGGG(+ACTCAC)GTACCTCTCCCCTTCAACCCCTGCCAGGACG NGG 10 8 17 8 24 0 100 5 10
    240605025121_8i1o AATGAAAGTGTGTTCTACTGTCAGTATAACTG(+ACTCAC)GATCCTCAGACCGGCACAGTGGGCTCCGGGA NGG 10 8 17 8 24 0 100 5 10
    240605025120_pgmr GAGTATCCCGGAGCCCACTGTGCCGGTCTGAG(+ACTCAC)GATCCAGTTATACTGACAGTAGAACACACTT NGG 10 8 17 8 24 0 100 5 10
    240605025118_wios ACGTGTCAGGTAGAGCTTTCTAAGTGAGCTGG(+ACTCAC)GATCCTGACTCACGTGTCATAGACTTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605025117_x8yy CTGCTCTTACAAGTCTATGACACGTGAGTCAG(+ACTCAC)GATCCCAGCTCACTTAGAAAGCTCTACCTGA NGG 10 8 17 8 24 0 100 5 10
    240605025115_tiub CCAGGCAGGCTGGTCCCTGCTGTGATTAATGG(+ACTCAC)GTACCCCAAAGTACAAACTAACGACAATGGA NGG 10 8 17 8 24 0 100 5 10
    240605025113_wwwy CAGCATCCATTGTCGTTAGTTTGTACTTTGGG(+ACTCAC)GTACCCATTAATCACAGCAGGGACCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605025112_6j1t GAGCGCAGTGGGATGAACTCACAGCAGTACTG(+ACTCAC)GTACCATTTTAGATTTCTTTCTTGCACTATA NGG 10 8 17 8 24 0 100 5 10