Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024714_0bz9 TGTTTAATGTGGGTATGATATTTTATATATAG(+ACTCAC)GATCCAATTCTCAGCAAGTGTATTCACATTT NGG 10 8 17 8 24 0 100 5 10
    240605024713_t0sd AAGCAGCTTTGTTGCTGCCTTTATGGACATGG(+ACTCAC)GTACCAATTGCTTTTTGTAATATGGTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605024711_1qaj TCACATTTTTACCATATTACAAAAAGCAATTG(+ACTCAC)GTACCCATGTCCATAAAGGCAGCAACAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605024710_mnn5 GGGTAGAATCCTTCCAGAAGCCATGCTTTCAG(+ACTCAC)GATCCACTGTGATTGTCCAGCAGGGCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605024709_o2bl TCATGAGCTAGCCCTGCTGGACAATCACAGTG(+ACTCAC)GATCCTGAAAGCATGGCTTCTGGAAGGATTC NGG 10 8 17 8 24 0 100 5 10
    240605024707_9h79 TCCTCCACCAAGTACAATACATAGTATCTCAG(+ACTCAC)GATCCAGTGACACCTATACATCTTGGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605024706_qfnt GGGACAGGGACCAAGATGTATAGGTGTCACTG(+ACTCAC)GATCCTGAGATACTATGTATTGTACTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605024705_0xzs GGTCCAAAGACTTGAGGGACTGTGTACTCAAG(+ACTCAC)GTACCAGGAATGGCTACGTCAGAACCCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605024703_d9fi TACAAAACAGGGTTCTGACGTAGCCATTCCTG(+ACTCAC)GTACCTTGAGTACACAGTCCCTCAAGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605024701_u6r1 CTACCCTCATGCCCCCTTTGCCTTTCCCTCGG(+ACTCAC)GATCCTGTTTGGGCTCCCTTTCCCCAAGCAA NGG 10 8 17 8 24 0 100 5 10