Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024728_8yxd GTAAACATTACACATGTCATTTTGTGGCACAG(+ACTCAC)GATCCCAAAATAAAATATCAAAGTAATCCCA NGG 10 8 17 8 24 0 100 5 10
    240605024726_df8n GAGATACATAAGGCACAACAGCTTTCCTTCAG(+ACTCAC)GATCCTTCTCTCTGTCCTAGAGTCTTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605024725_9q2y CTACCGTCTGAAGACTCTAGGACAGAGAGAAG(+ACTCAC)GATCCTGAAGGAAAGCTGTTGTGCCTTATGT NGG 10 8 17 8 24 0 100 5 10
    240605024724_6jrh CATCAACTGCAAACGTGAGGGGAGAAAGCATG(+ACTCAC)GTACCCAACCAAATTCCACATTTCAGCAATA NGG 10 8 17 8 24 0 100 5 10
    240605024722_tmpq TGAAGTATTGCTGAAATGTGGAATTTGGTTGG(+ACTCAC)GTACCATGCTTTCTCCCCTCACGTTTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605024721_s5k6 TTTATAAGGAAAAGACTCAAGGTATAAGCTCG(+ACTCAC)GATCCCTAACCCTGTGCTATGTCATCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605024719_hudt GTGAGAGAAAGATGACATAGCACAGGGTTAGG(+ACTCAC)GATCCGAGCTTATACCTTGAGTCTTTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605024718_85jq TCAGTCCAGAACTAAAAATCAATGTGAAAATG(+ACTCAC)GATCCTCACCTTGAAAGACAAGTGTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240605024717_hsfm AGAAAGAAGTCACACTTGTCTTTCAAGGTGAG(+ACTCAC)GATCCATTTTCACATTGATTTTTAGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605024715_nf92 AGAAAAAATGTGAATACACTTGCTGAGAATTG(+ACTCAC)GATCCTATATATAAAATATCATACCCACATT NGG 10 8 17 8 24 0 100 5 10