Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024826_uvz8 CCGGCTCCCACCTCTGCGTGGCCTGTTGTCAG(+ACTCAC)GATCCACAGGTCCCCTCTCCCTCAGCAGACA NGG 10 8 17 8 24 0 100 5 10
    240605024825_idj5 TGTATCAATACTGTTTTACAAATTGGAAGTGG(+ACTCAC)GTACCTCAGTCCAGAGCTGAGCACCCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605024823_9qls GATTGATGCAGGGTGCTCAGCTCTGGACTGAG(+ACTCAC)GTACCCACTTCCAATTTGTAAAACAGTATTG NGG 10 8 17 8 24 0 100 5 10
    240605024822_9ad0 ATAATACATATCACTTCCCTGCCCCTGAATAG(+ACTCAC)GTACCATCTTATTTTTATTAATAGAGTACTA NGG 10 8 17 8 24 0 100 5 10
    240605024821_muw0 CAAACTAGTACTCTATTAATAAAAATAAGATG(+ACTCAC)GTACCTATTCAGGGGCAGGGAAGTGATATGT NGG 10 8 17 8 24 0 100 5 10
    240605024820_lp11 ATTCCTGGTATCTGAAGCCAAAAGTGGTCATG(+ACTCAC)GATCCCCTGAGAATGGAGTTTGAGATATCTG NGG 10 8 17 8 24 0 100 5 10
    240605024818_19vu GCTCACAGATATCTCAAACTCCATTCTCAGGG(+ACTCAC)GATCCATGACCACTTTTGGCTTCAGATACCA NGG 10 8 17 8 24 0 100 5 10
    240605024817_j292 GAACACGAGGTCAGAAGCTTCTCTTCTATGTG(+ACTCAC)GATCCTTGAACTAGTCCGGCCGGCGTGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605024815_itvz GTTCGACTTCACGCCGGCCGGACTAGTTCAAG(+ACTCAC)GATCCACATAGAAGAGAAGCTTCTGACCTCG NGG 10 8 17 8 24 0 100 5 10
    240605024813_vfzp AACATTTCTGACTACATTATAAAGCACCACAG(+ACTCAC)GTACCAGGCGATTTCTGTATGCTTATTTTGA NGG 10 8 17 8 24 0 100 5 10