Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024857_senx GAGCAATATTTACACCATAAATCCAGATCTTG(+ACTCAC)GTACCCCACCCCACCACACCCCACCCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240605024856_cnh2 CATAAAGTGACAGGAGCCTGGGTAAAGACTTG(+ACTCAC)GTACCAACATTGGATGCTGTCAAGGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605024854_6mb1 GCCAGGGAGAGCCTTGACAGCATCCAATGTTG(+ACTCAC)GTACCAAGTCTTTACCCAGGCTCCTGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605024853_i05f ACCGGTGAAGAAAGAAGTCTCTCTTCAACACG(+ACTCAC)GATCCAGGCAGCCAGTCTTCACCTGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240605024851_b3kj CACCCTGTGCTCAGGTGAAGACTGGCTGCCTG(+ACTCAC)GATCCGTGTTGAAGAGAGACTTCTTTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605024850_r9zl TTTATATATATTAACTTTCATTGCATTTTTTG(+ACTCAC)GTACCTTCACAACTGGTGTGAAATAAATTCA NGG 10 8 17 8 24 0 100 5 10
    240605024849_5a4f TGATGTGAATTTATTTCACACCAGTTGTGAAG(+ACTCAC)GTACCAAAAAATGCAATGAAAGTTAATATAT NGG 10 8 17 8 24 0 100 5 10
    240605024847_3nsn CAATAAAGGAACAAAAGAACCCAGCAATCCAG(+ACTCAC)GTACCATGATCTTGCTCAAGTAGAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605024845_4xfo GAGGGCATCTCTTCTACTTGAGCAAGATCATG(+ACTCAC)GTACCTGGATTGCTGGGTTCTTTTGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605024844_tkmw TTTAAGTTTCATAACTGTACTAGAGATGGCAG(+ACTCAC)GATCCTGCCCAGCACAGTCTTTCCGTTTAAT NGG 10 8 17 8 24 0 100 5 10