Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024911_u2yt ATACCCACATGGTGGTTCACAGCCATCCAGAG(+ACTCAC)GATCCTGTGCTTTCCTGTGACCTCTGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605024909_hk3p CAGTCCCAGCACCCAGGAACTTGAGGTAGGAG(+ACTCAC)GATCCTAAGTTCAAAGCTGGCCTTGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240605024908_foe1 TGCTAAATAGCCAAGGCCAGCTTTGAACTTAG(+ACTCAC)GATCCTCCTACCTCAAGTTCCTGGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605024907_3xd8 TCCTATAGTTTATTCCTCAGAAGAGGTTTCTG(+ACTCAC)GTACCTTCCTTCTCCAGACCGTCTAAGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605024905_yihs ACAGGGCACCTTAGACGGTCTGGAGAAGGAAG(+ACTCAC)GTACCAGAAACCTCTTCTGAGGAATAAACTA NGG 10 8 17 8 24 0 100 5 10
    240605024904_modq CTCCCAGCTCCTGCATCTCCTTCCCTGCACAG(+ACTCAC)GTACCACTTGGCCCCATTGAGGCTGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605024903_rvra GCTGGTCCTGCCAGCCTCAATGGGGCCAAGTG(+ACTCAC)GTACCTGTGCAGGGAAGGAGATGCAGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605024901_o54h CCAAACGCTCACTGTCAGAAAGTGGTAGTGAG(+ACTCAC)GATCCTGAGTGAAGCTGGAGAACAGAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605024900_du7q GGGTGTAGGCTCTGTTCTCCAGCTTCACTCAG(+ACTCAC)GATCCTCACTACCACTTTCTGACAGTGAGCG NGG 10 8 17 8 24 0 100 5 10
    240605024858_2cja TTTTTTAAAGGGGTGGGGTGTGGTGGGGTGGG(+ACTCAC)GTACCAAGATCTGGATTTATGGTGTAAATAT NGG 10 8 17 8 24 0 100 5 10