Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024644_ewnu AACTGGTAGGTACATGGTTGCTTGCCTGCTTG(+ACTCAC)GTACCATCTCTCAAGGGAAAATACAAAATCT NGG 10 8 17 8 24 0 100 5 10
    240605024642_k2y2 AATTGGGTTTTGTCTTCTTTAAAAACAAATAG(+ACTCAC)GTACCGTAAGCATTTTCTGCTGACGACTGCC NGG 10 8 17 8 24 0 100 5 10
    240605024641_8vlp CTTAAGGCAGTCGTCAGCAGAAAATGCTTACG(+ACTCAC)GTACCTATTTGTTTTTAAAGAAGACAAAACC NGG 10 8 17 8 24 0 100 5 10
    240605024640_b93a TGAATAAGAACCAAACTGAGTATTATAGTGAG(+ACTCAC)GATCCTTGACCACTTAGTAGACCTCAGCAAA NGG 10 8 17 8 24 0 100 5 10
    240605024639_qubp AGAATTTTGCTGAGGTCTACTAAGTGGTCAAG(+ACTCAC)GATCCTCACTATAATACTCAGTTTGGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605024637_e0qp ACCTTCGCAAAAACTTAAATCATGGAATGGCG(+ACTCAC)GTACCACGGTAGAACACTGCAGCTGTCGCCA NGG 10 8 17 8 24 0 100 5 10
    240605024635_j6gg AACCCTGGCGACAGCTGCAGTGTTCTACCGTG(+ACTCAC)GTACCGCCATTCCATGATTTAAGTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240605024633_0owx GAATCGACCACCATACCACAGTAACGCCCCTG(+ACTCAC)GATCCCTGGCTGCCCACCCACTCTAAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605024630_5c2o CAGGATAGCCTTAGAGTGGGTGGGCAGCCAGG(+ACTCAC)GATCCAGGGGCGTTACTGTGGTATGGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605024629_w4ne TACAAACACTAAGCATAAAAGCACATGTGGTG(+ACTCAC)GTACCTGGGTATCTAGTTCTTACGGAATAAT NGG 10 8 17 8 24 0 100 5 10