Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023004_kady GGTAGGCCAGCAAGGGACAGGACTATTGCCTG(+ACTCAC)GTACCTTCCCCAGGTGTGCTTATTCATGAGT NGG 10 8 17 8 24 0 100 5 10
    240605023003_r10m TCACACATTCCAGAGACATCAACCCTGCAGTG(+ACTCAC)GATCCAGTGTGATTTCCAGACTCTCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605023001_ybyo TTTTGTGCCTGGAGAGTCTGGAAATCACACTG(+ACTCAC)GATCCACTGCAGGGTTGATGTCTCTGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605022958_qe0o ACATCAGGCACCAGTGAAGATACATCCCCCTG(+ACTCAC)GATCCCATTGATGAGGACTCTAAAAGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605022957_n7k7 CTTCTATCCCCTTTTAGAGTCCTCATCAATGG(+ACTCAC)GATCCAGGGGGATGTATCTTCACTGGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605022955_h0cz TCAAATCGATTTTGTTCTTGATTTTTATGAAG(+ACTCAC)GATCCATCTCTGTGTATTGAGGGGTAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605022954_4zky AAATCATTTTTACCCCTCAATACACAGAGATG(+ACTCAC)GATCCTTCATAAAAATCAAGAACAAAATCGA NGG 10 8 17 8 24 0 100 5 10
    240605022953_1m5w GCTAGAACTTACAGAGGCTGTGGTTGATCTGG(+ACTCAC)GTACCATGTGCCATGATGTTTTACTGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605022951_e0gz TAAAAGCTGGCAGTAAAACATCATGGCACATG(+ACTCAC)GTACCCAGATCAACCACAGCCTCTGTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605022949_driq AGATTAAAACCCTCTGCAAGCGCACAGCCTTG(+ACTCAC)GTACCGCCTCTCATCTCAGTTAACAGTTAGA NGG 10 8 17 8 24 0 100 5 10