Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022859_4p0o CTCTGCTTGTCTTGATGCTGTGGTGGCAGATG(+ACTCAC)GATCCAGGCCCTGCTGTGCCCAGTGACCCTT NGG 10 8 17 8 24 0 100 5 10
    240605022858_g31j ACCACAGTCTCACAAACAGAGAGGTAAACACG(+ACTCAC)GATCCTTGCTTGCCCTCGGCTGGCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605022857_h4x6 CGAGAGGAGAAAGCCAGCCGAGGGCAAGCAAG(+ACTCAC)GATCCGTGTTTACCTCTCTGTTTGTGAGACT NGG 10 8 17 8 24 0 100 5 10
    240605022855_2xen AGGGTGCCCATGGATGCCACAGGAAGGCATGG(+ACTCAC)GATCCCTTGGAGCTTGAGTCCCAGGCAATTT NGG 10 8 17 8 24 0 100 5 10
    240605022853_u1sk CACCTAAATTGCCTGGGACTCAAGCTCCAAGG(+ACTCAC)GATCCCATGCCTTCCTGTGGCATCCATGGGC NGG 10 8 17 8 24 0 100 5 10
    240605022852_j8dn CTAGGGTTTTCACCTAGCCTCTGGTAGCTAAG(+ACTCAC)GATCCCACCACATCCTTCATATTCCCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605022850_62e4 TACAGTGACTGGGAATATGAAGGATGTGGTGG(+ACTCAC)GATCCTTAGCTACCAGAGGCTAGGTGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605022848_igz9 GTAGCTCTAGAAACATTTTTAAAAGGTGAGGG(+ACTCAC)GATCCCACCTCATCCCATTTTTTAAGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605022846_uy7j ACCATTTACACTTAAAAAATGGGATGAGGTGG(+ACTCAC)GATCCCCTCACCTTTTAAAAATGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605022845_u5w6 AGATGCACTCATCGCTTAAGGTGATGCAGTTG(+ACTCAC)GATCCTATTTCTTACGTTTCTCTGCTGTTCC NGG 10 8 17 8 24 0 100 5 10