Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022843_ezyf TCTAAGGAACAGCAGAGAAACGTAAGAAATAG(+ACTCAC)GATCCAACTGCATCACCTTAAGCGATGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605022841_7jzf GCCACTGCTGCTTGGTGGCTCTCTCCCGAGGG(+ACTCAC)GTACCATCCTGCCCTCCCATGGGTCTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605022840_sw36 CTTGCTCCAAAGACCCATGGGAGGGCAGGATG(+ACTCAC)GTACCCCTCGGGAGAGAGCCACCAAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605022838_x95e TCATCGAGGCTGGCCTTGAATTCCTAATCCTG(+ACTCAC)GTACCTCCACCTCCCATGTGCTAGGATCACA NGG 10 8 17 8 24 0 100 5 10
    240605022836_r04u ATGCCTGTGATCCTAGCACATGGGAGGTGGAG(+ACTCAC)GTACCAGGATTAGGAATTCAAGGCCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605022835_bb4s TGCTCTTGCAAAGGACCAGAGTTTGATTCCTG(+ACTCAC)GTACCTGTGTCAGGAAGCTCACAGCCACCTG NGG 10 8 17 8 24 0 100 5 10
    240605022834_p5n1 AGTCACAGGTGGCTGTGAGCTTCCTGACACAG(+ACTCAC)GTACCAGGAATCAAACTCTGGTCCTTTGCAA NGG 10 8 17 8 24 0 100 5 10
    240605022832_m5wt AAAAGAACCATATTCAGGAAAGCCCACTATCG(+ACTCAC)GTACCAGTTTTTCAACATCCTCTGATCATGG NGG 10 8 17 8 24 0 100 5 10
    240605022831_1qv1 TAGAGCCATGATCAGAGGATGTTGAAAAACTG(+ACTCAC)GTACCGATAGTGGGCTTTCCTGAATATGGTT NGG 10 8 17 8 24 0 100 5 10
    240605022830_1dwb TACAAGATCAACTGCTGGCTCCATAGCTTCTG(+ACTCAC)GATCCAGTGTCCCTTTCCCTTCAGAAGCCTG NGG 10 8 17 8 24 0 100 5 10