Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022829_ifjs GTGTTCAGGCTTCTGAAGGGAAAGGGACACTG(+ACTCAC)GATCCAGAAGCTATGGAGCCAGCAGTTGATC NGG 10 8 17 8 24 0 100 5 10
    240605022827_l1bc CTCCTGTGGCCTCTCCAGCAGGAGACCCACAG(+ACTCAC)GATCCCATGCAATGGCTGCTTTGCTCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605022825_u40a GCCTACTGAGGAGCAAAGCAGCCATTGCATGG(+ACTCAC)GATCCTGTGGGTCTCCTGCTGGAGAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605022824_1euy GCTAAATGGATGCTTCAGTCCATGGCTGAGAG(+ACTCAC)GTACCGCCTGCACCAGCAAAGTGGAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605022822_wy2v AGCTTCAGCACTCCACTTTGCTGGTGCAGGCG(+ACTCAC)GTACCTCTCAGCCATGGACTGAAGCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240605022820_acwo TAGCTTTGTTACATCCTAGCAAGATATTCCTG(+ACTCAC)GATCCCTGCTGCGCATTCTGATGTGAATACC NGG 10 8 17 8 24 0 100 5 10
    240605022819_qevb ACCTTGGTATTCACATCAGAATGCGCAGCAGG(+ACTCAC)GATCCAGGAATATCTTGCTAGGATGTAACAA NGG 10 8 17 8 24 0 100 5 10
    240605022818_oep2 ATATTTTTTCACCTAATCTCGGGGTCTTACTG(+ACTCAC)GTACCCTCACCCAAGTGCCTATGAAGCACGA NGG 10 8 17 8 24 0 100 5 10
    240605022816_oeqp GAACCTCGTGCTTCATAGGCACTTGGGTGAGG(+ACTCAC)GTACCAGTAAGACCCCGAGATTAGGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605022815_wryr GTGACTCACTCTCAGTGGTACTGAGGAGGCAG(+ACTCAC)GATCCTCATCTTCCTGTACTGCCCACCTGAC NGG 10 8 17 8 24 0 100 5 10