Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022916_2o2d ACAGCGGCAGTGCGATACCATCAGCTGCTTAG(+ACTCAC)GATCCGTGTGATTAACAGTGTCTTAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605022913_aqda CTCGAAGAGTTTAGCATTTATTAACTCCCAGG(+ACTCAC)GATCCTAAACTCTTTGCTGATGGAATTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605022912_hnxr AGATTGGGCAATTCCATCAGCAAAGAGTTTAG(+ACTCAC)GATCCCTGGGAGTTAATAAATGCTAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240605022910_gocx CTCGCTCAAACAGTCCTACTCACAAACAGGAG(+ACTCAC)GTACCATATCCTACATCCTTTGGCTTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605022909_a23p CACTGTGGAAAAGCCAAAGGATGTAGGATATG(+ACTCAC)GTACCTCCTGTTTGTGAGTAGGACTGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605022907_dqhe TGACTGAAGACAAGGTGGATCTTAGCTAATAG(+ACTCAC)GATCCTCCCAGGGTTGTCAAGCGGGATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605022906_nz2t ACTCTCCAGATCCCGCTTGACAACCCTGGGAG(+ACTCAC)GATCCTATTAGCTAAGATCCACCTTGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605022904_51yi AAGTCTGCGCACACACCTTCTCTGGTCTCTGG(+ACTCAC)GATCCAGGAGGTCAGCGAGCATCCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605022902_wkis TAGGAAGGGAGGGGATGCTCGCTGACCTCCTG(+ACTCAC)GATCCCAGAGACCAGAGAAGGTGTGTGCGCA NGG 10 8 17 8 24 0 100 5 10
    240605022900_6j2n CTTAGAAGGGTCACTGGGCACAGCAGGGCCTG(+ACTCAC)GATCCATCTGCCACCACAGCATCAAGACAAG NGG 10 8 17 8 24 0 100 5 10