Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020510_5ka9 AAAGAAAGTCCATTTCTGTGAGACCCATTCCG(+ACTCAC)GTACCCATGTGTCCTCCCTTCACTCATGCAG NGG 10 8 17 8 24 0 100 5 10
    240605020508_j6j8 AGGCTCCACACACCAGGTATTGCTGATTCCAG(+ACTCAC)GTACCACCCTTGACATGTGTGTCTGAGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605020506_gtpb AGGCTGGGACTCAGACACACATGTCAAGGGTG(+ACTCAC)GTACCTGGAATCAGCAATACCTGGTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605020504_yamh CAGCTTTGTCAACAGTTCTGTATCCATACCTG(+ACTCAC)GTACCCTCGGCCCCGCTTCCGAGTCGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605020501_ulq3 AAGTGAAAAGCGACTCGGAAGCGGGGCCGAGG(+ACTCAC)GTACCAGGTATGGATACAGAACTGTTGACAA NGG 10 8 17 8 24 0 100 5 10
    240605020459_exjb AGAAGACACCCAGGGTGACGTCTCATCCTCAG(+ACTCAC)GATCCCCCACGTCTGCTGCCATCACAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240605020457_3f1d GGGATGCGGCTGTGATGGCAGCAGACGTGGGG(+ACTCAC)GATCCTGAGGATGAGACGTCACCCTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605020456_vr67 ACAGGGGTAAAGGTTAGACAGTGGCAACTTAG(+ACTCAC)GATCCTGTTAGGGTCTGGCCCATGTTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605020454_xn8j TGGGAGTTAAAACATGGGCCAGACCCTAACAG(+ACTCAC)GATCCTAAGTTGCCACTGTCTAACCTTTACC NGG 10 8 17 8 24 0 100 5 10
    240605020453_nr4q TAACTCATGCCCAGACACTGATGGAGGGGCAG(+ACTCAC)GTACCCCCAAAACACCAAGCCCTAAGAGGCC NGG 10 8 17 8 24 0 100 5 10