Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020417_jrfz GAAATGACAAGAGGGAAAACAGCTGGTGGCGG(+ACTCAC)GATCCCAGTCAGCCTTCCCGCTCTGCACCTG NGG 10 8 17 8 24 0 100 5 10
    240605020415_k17u GGGTGGCCAGGAGCAGCAGAGGTGAAGGGATG(+ACTCAC)GATCCACCTGCGGGTCTCTGTGGTTACCGTA NGG 10 8 17 8 24 0 100 5 10
    240605020414_r09j ATCACTACGGTAACCACAGAGACCCGCAGGTG(+ACTCAC)GATCCATCCCTTCACCTCTGCTGCTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605020413_avc2 CTCTGCATCCATCTTGATTAAAGATGGGTGTG(+ACTCAC)GATCCTCTCCCACATCCTAGGGTGTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605020411_fb27 ATGGCTGCCCCACACCCTAGGATGTGGGAGAG(+ACTCAC)GATCCACACCCATCTTTAATCAAGATGGATG NGG 10 8 17 8 24 0 100 5 10
    240605020410_ldb2 CTCCCTTCAGCACGGTAGGTCTTTGCATCTAG(+ACTCAC)GATCCCCGCCCCAGACCGCCTGTGAGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605020408_h33y CTTTGCTCTGCTCACAGGCGGTCTGGGGCGGG(+ACTCAC)GATCCTAGATGCAAAGACCTACCGTGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605020407_q2g1 TGGTAGCCTGTGGTCAGGCAGCCCTTGTAATG(+ACTCAC)GATCCCTACCTTCTTGTAAAGTCTCTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605020405_60la ACACATTTTTAGAGACTTTACAAGAAGGTAGG(+ACTCAC)GATCCATTACAAGGGCTGCCTGACCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605020403_84yv AATACCCTCTTTCTGACCTCCACAGGCACAGG(+ACTCAC)GTACCCATGTGGTATACATATATACACAAGG NGG 10 8 17 8 24 0 100 5 10