Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020331_ev7i CAGAAGATGCTAAGCTGTCATAGGTGGAGCAG(+ACTCAC)GATCCTAAGGACGCACAATATCAGTTGTGCG NGG 10 8 17 8 24 0 100 5 10
    240605020329_p4hq CAGCACGCACAACTGATATTGTGCGTCCTTAG(+ACTCAC)GATCCTGCTCCACCTATGACAGCTTAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605020328_5lbx TCGAGATCCAGTGGAAGCTAGAAAGTGCACAG(+ACTCAC)GATCCCAGAGCTACAGCCTGGCAGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605020326_lxpv AGAGGATGACTTCTGCCAGGCTGTAGCTCTGG(+ACTCAC)GATCCTGTGCACTTTCTAGCTTCCACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605020325_juap CCGCTTTGTGAGAGCATCAGGCCCTGTGTAGG(+ACTCAC)GATCCCCAGCAGTAACACTTGTCAGTTTACT NGG 10 8 17 8 24 0 100 5 10
    240605020323_0jrd GGACCAGTAAACTGACAAGTGTTACTGCTGGG(+ACTCAC)GATCCCTACACAGGGCCTGATGCTCTCACAA NGG 10 8 17 8 24 0 100 5 10
    240605020322_7qlo TCGAGATCCAGTGGAAGCTAGAAAGTGCACAG(+ACTCAC)GATCCCAGAGCTACAGCCTGGCAGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605020320_8q7a AGAGGATGACTTCTGCCAGGCTGTAGCTCTGG(+ACTCAC)GATCCTGTGCACTTTCTAGCTTCCACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605020319_dz36 GGGCTGTCTCTGACCCTATTACCTGCCTTTGG(+ACTCAC)GATCCATTCCCCCTCCTGCATCGTCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240605020317_dvwe TACTAATGTTGGACGATGCAGGAGGGGGAATG(+ACTCAC)GATCCCAAAGGCAGGTAATAGGGTCAGAGAC NGG 10 8 17 8 24 0 100 5 10