Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020301_5yr3 AAGTGACAAGCTGACTGTGGCATTGCACACTG(+ACTCAC)GATCCAAAGAGAAGGTTGTAAAAGAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605020300_7p0o ACCAACCCAGCTCTTTTACAACCTTCTCTTTG(+ACTCAC)GATCCAGTGTGCAATGCCACAGTCAGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605020258_21nc ATTGGGGAGTGGGAGGGGGGACAGCACAGGTG(+ACTCAC)GATCCAGAAATAGGGGGAAGGCTGCAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605020257_3zph CTCATTTGCTTGCAGCCTTCCCCCTATTTCTG(+ACTCAC)GATCCACCTGTGCTGTCCCCCCTCCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240605020256_hv5i TACTACAGACTCACACAGCCTTGTGTTAGGTG(+ACTCAC)GTACCAATCTGTGTTTATTGAAAACCTACGT NGG 10 8 17 8 24 0 100 5 10
    240605020254_ihib GCTGGACGTAGGTTTTCAATAAACACAGATTG(+ACTCAC)GTACCACCTAACACAAGGCTGTGTGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605020252_vaq6 GGCAGTGGCCTGCAGCCTGCCCTCACCAAGGG(+ACTCAC)GATCCAGGCCAGAAGTTCCTTCAGCCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605020250_kteo GGAGGAGGCTGGCTGAAGGAACTTCTGGCCTG(+ACTCAC)GATCCCCTTGGTGAGGGCAGGCTGCAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605020249_xqri ACAACATACCAAAGGAATCTGCAATCCTGCAG(+ACTCAC)GATCCAAATAACCAATTTTATTAGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605020248_1kwn GTCTCAGAGCCTCTAATAAAATTGGTTATTTG(+ACTCAC)GATCCTGCAGGATTGCAGATTCCTTTGGTAT NGG 10 8 17 8 24 0 100 5 10